“We aren’t going to get sick, are we?” my roommate Brett asked me. He cringed as I knelt down and stuffed a plate of E. coli bacteria—which came as part of the DIY CRISPR–Cas9 kit I bought online—into our fridge next to cartons of eggs, strawberry jam, bottles of beer and a block of cheese.
“No, we won’t. The label says ‘non-pathogenic,’” I replied, trying to sound assuring. But honestly, I had no clue what I was doing. I nudged all the food up against the fridge wall, and left a two-inch border around the plate of living cells—a no man’s land between the microbes and our dinner. A couple inches probably would not stop the bugs, but I figured it couldn’t hurt.
CRISPR–Cas9 (or CRISPR, for short) has given scientists a powerful way to make precise changes to DNA—in microbes, plants, mice, dogs and even in human cells. The technique may help researchers engineer drought-resistance crops, develop better drugs, cure genetic disorders, eradicate infectious diseases and much more. Ask any biologist, and they’ll likely tell you that CRISPR is revolutionary. It’s cheap and effective, and in many cases, it works much better than older methods for making genetic modifications. Biologists will also tell you that CRISPR is very easy to use. But what does “easy to use” mean?
I am not a DIY scientist, much less a professional scientist. You won’t find me swabbing my cheek cells for DNA or tinkering with yeast in a lab on the weekend. But I wondered: Is CRISPR so easy that even amateurs like me can make meaningful contributions to science? And also, does this new technique make gene editing so accessible that we need to worry about DIY scientists cooking up pandemic viruses in their basements? If you Google ‘DIY CRISPR,’ stories such as “What Happens If Someone Uses this DIY Gene Hacking Kit to Make Mutant Bacteria?” pop up.
I attempted to find answers to all these questions myself, starting with the plate of bacteria in the kitchen of my San Francisco apartment.
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