Having a microscope that can magnify and enhance the tiniest details of biological structures can reveal a world beyond the limits of conventional resolution. This is precisely what enhanced super-resolution radial fluctuations (eSRRF) bring to the field of microscopy.

In the field of microscopy, two powerful techniques, light, and electron microscopy, provide complementary information on biological samples across broad size regimes. However, a gap between light and electron microscopy prompts the physicists to search for ways to overcome the resolution barrier.

Over the last two decades, super-resolution microscopy (SRM) developments have allowed the unprecedented observation of nanoscale structures in biological systems. SRM refers to a series of fluorescence imaging techniques in optical microscopy that enables images to have higher resolutions than those imposed by the diffraction limit.

Different SRM methods have evolved and are being widely applied in biomedical research. Some techniques can have nanometer-scale molecular resolution, while others are geared towards volumetric 3D multi-color or fast live-cell imaging.

In particular, super-resolution radial fluctuation (SRRF) is a versatile technique that achieves live-cell SRM on a wide range of microscopy platforms. As a computational approach to SRM, SRRF is highly accessible to life science researchers since it allows using common microscopes. Users are enabled to generate super-resolution images using the same equipment and methods they routinely employ for their studies without preparing specialized samples or reagents.

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